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245 1 0 _aWhole-Body Regeneration
_h[electronic resource] :
_bMethods and Protocols /
_cedited by Simon Blanchoud, Brigitte Galliot.
250 _a1st ed. 2022.
264 1 _aNew York, NY :
_bSpringer US :
_bImprint: Humana,
_c2022.
300 _aXVII, 685 p. 131 illus., 123 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2450
505 0 _aThe Hazards of Regeneration: From Morgan’s Legacy to Evo-Devo -- Studying Regeneration in Ascidians: An Historical Overview -- Studying Protista WBR and Repair using Physarum polycephalum -- Studying Porifera WBR using the Calcerous Sponges Leucosolenia -- Studying Ctenophora WBR using Mnemiopsis leidyi -- Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerens -- Collecting and Culturing Kamptozoans for Regenerative Studies -- Collecting and Culturing Bryozoans for Regenerative Studies -- Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride -- Studying Annelida Body Regeneration under Environmental Stress in Diopatra neapolitana -- Studying Annelida Regeneration using Platynereis dumerilii -- Collecting and Culturing Lineus sanguineus to Study Nemertea WBR -- Studying Xenacoelomorpha WBR using Isodiametra pulchra -- Studying Echinodermata Arm Explant Regeneration using Echinaster sepositus -- Studying Hemichordata WBR using Ptychodera flava.-Studying Tunicata WBR using Botrylloides anceps -- In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis -- Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi -- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals -- Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry -- Non-Invasive Intra-Vascular Micro-Transfusion in Colonial Tunicates -- Gene Manipulation in Hydractinia -- Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis -- Monitoring Telomere Maintenance during Regeneration of Annelids -- Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians -- Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignano -- RNAi Screening to Assess Tissue Regeneration in Planarians -- Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq) -- Assessing Chromatin Accessibility during WBR in Acoels -- Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensis -- Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialis -- Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. Regeneration -- Studying Mechanical Oscillations during Whole-Body Regeneration in Hydra -- Combining RNAi-Mediated β-catenin Inhibition and Reaggregation to Study Hydra Whole-Body Regeneration -- Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostelle vectensis -- Formalizing Phenotypes of Regeneration.
506 0 _aOpen Access
520 _aThis Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.
650 0 _aRegenerative medicine.
650 0 _aStem cells.
650 1 4 _aRegenerative Medicine and Tissue Engineering.
650 2 4 _aStem Cell Biology.
700 1 _aBlanchoud, Simon.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aGalliot, Brigitte.
_eeditor.
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
710 2 _aSpringerLink (Online service)
773 0 _tSpringer Nature eBook
776 0 8 _iPrinted edition:
_z9781071621714
776 0 8 _iPrinted edition:
_z9781071621738
776 0 8 _iPrinted edition:
_z9781071621745
830 0 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2450
856 4 0 _uhttps://doi.org/10.1007/978-1-0716-2172-1
912 _aZDB-2-PRO
912 _aZDB-2-SOB
999 _c80342
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945 _a1
_bMadushi Gamage
_c1
_dMadushi Gamage